The neurophysiological basis of BOLD contrast mechanism in fMRI is not fully understood. Therefore we started to investigate the role of different neuromodulators and channel blockers on the neural and hemodynamic responses. We first report the effects of local injection of Lidocaine, a reversible sodium channel blocker, in primary visual cortex (V1) of anesthetized monkeys. The effects were assessed by simultaneous intracortical recordings and fMRI. We examined BOLD responses in regions of interest defined by independent localizer scans, and assessed the spatial effect of the blocker at varying distances from the injection site. Neuroimaging was performed in a 4.7 Tesla Scanner (Bruker, Germany). Recorded were spiking activity and local field potentials. V1 was stimulated by rotating polar checkerboard stimulus (blocks by 30 sec stimulus, 30 sec blank, 37 repetitions). 300 μm to the recording electrode we injected Lidocaine (2% solution) with a precision pump (M6 VICI, USA). Applied quantities (8-25 μl) and flow rates (0.8-4 μl /min) were monitored by a precision flow meter (Sensirion, Switzerland). Consistent with previous reports, Lidocaine induced reliable decreases in neuronal activity at the injection site. In addition, we observed clear decreases in BOLD activity. The largest effect on both signals was observed closest to the injection site and decreased with increasing distance. The effect was reversible for both signals with a recovery time of 20-30 minutes. Injection of saline (0.9%), to rule out nonspecific effects, showed no change in neuronal or BOLD signals. The findings suggest a close coupling between stimulus-evoked neuronal activity and the BOLD signal. This allows for a better quantification of the primarily interesting part of the BOLD signal involved in neuronal processing as we now can distinguish b